Monolysocardiolipin: improved preparation with high yield.
نویسندگان
چکیده
A simple, high-yielding preparation of monolysocardiolipin (MLCL) by phospholipase A2 hydrolysis of cardiolipin (CL) in methanol on a semi-preparative scale is described. In methanol, phospholipase A2 preferentially hydrolyzes CL to MLCL. This selectivity results in ∼80% yield of MLCL. The synthesized MLCL and dilysocardiolipin were characterized by NMR and ESI-MS/MS. Only the sn-2 position of CL was hydrolyzed by phospholipase A2 in methanol.
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عنوان ژورنال:
- Journal of lipid research
دوره 52 2 شماره
صفحات -
تاریخ انتشار 2011